Microbiology

Achromobacter xyloxocidans

A170: Achromobacter xyloxocidans a new emerging microorganism in cystic fibrosis.
C.R. Hansen, T. Pressler, C. Koch, H.K. Johansen, N. Hoiby
Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark

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Aspergillus fumigatus and other Fungal infections

A217: Aspergillus fumigatus an airway pathogen in CF – not only ABPA
K.G. Brownlee , M. Denton , S. Conway
Regional Paediatric Cystic Fibrosis Centre, St. James’s University Hospital, Leeds, United Kingdom, Department of Microbiology, Leeds Teaching Hospitals, Leeds, United Kingdom

A236: Report of a case of Paecilomyces lilacinus (P. lilacinus) infection in a CF patient.
G.A. Loeuille , F. Delepoulle , I. Joly-Durand , V. Montagne
Cystic fibrosis center, Dunkerque, France, Laboratory of parasitology and mycology, Lille, France

A216: Frequency of sensitization to Aspergillus fumigatus and its influence to the patients with cystic fibrosis (CF)
I. Loukou, V. Talanti, M. Liatsis, M. Kanariou, S. Doudounakis
CF Centre, Aghia Sophia Children’s Hospital, Athens, Greece

A177: SAP virulence gene expression of C. albicans sputum isolates from CF patients.
F.-M. Muller , S. Salvenmoser , R. Hess , M. Wiebel , C. Gil-Lamaignere
Pad. Pneumolgie, Mukoviszidose u. Infektiologie, Heidelberg, Germany, CF- Ambulanz, Thoraxklinik, Heidelberg, Germany

A171: Risk factors for allergic bronchopulmonary aspergillosis and sensitisation to Aspergillus fumigatus in patients with cystic fibrosis.
N. Ritz, R.A. Ammann, C. Casaulta, F. Schoni-Affolter, M.H. Schoni
Department of Paediatrics, Universitiy of Berne, Berne, Switzerland

A169: Scedosporium apiospermum sputum isolation in cystic fibrosis.
M. Wickremasinghe, K. Gyi, A. Hall, M. Hodson
Royal Brompton Hospital, London, United Kingdom

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Burkholderia Cepacia and other Burkholderia species

A162: Investigation of the interaction of B. cepacia complex with lung epithelial cells in vitro.
CE Duff , T Mullen , PG Murphy , M Callaghan , S McClean
Institute of Technology, Tallaght, Dubline, Ireland Adelaide, Meath and Incorporating National Children’s Hospital, Tallaght, Dublin, Ireland

A128: Burkholderia cepacia-an infrequent cause of a sepsis in a non-CF patient
A. Holcikova , P. Drevinek , D. Burgetova
Pediatric Infectious Clinic, Masaryk University, Brno, Czech Republic, Pediatric Clinic, Charles University, Prague, Czech Republic, Microbiology Department, University Hospital, Brno, Czech Republic

A6: The role of insertion sequences in the genomic plasticity of the Burkholderia cepacia complex
D.T. Kenna , H. Yesilkaya , K.J. Forbes , V.A. Barcus , J.R.W. Govan
University of Edinburgh, Edinburgh, United Kingdom, University of Aberdeen, Aberdeen, United Kingdom

A132: Burkholderia cepacia infection and clinical course in cystic fibrosis patients.
L. Pereira, S. Correia, C. Nascimento, T. Bandeira, C. Barreto.
Santa Maria Hospital, Lisbon, Portugal, Instituto Superior Tecnico, Lisbon, Portugal

A126: Clinical follow-up of 122 Italian cystic fibrosis patients with B. cepacia complex infection
G. Taccetti , M. Ambroni , B. Assael , A. Cosimi , D. Costantini , M.L. Furnari , R. Gagliardini , G.L. Grzinchich , A. Manca , A. Negri , G. Pizzamiglio , F. Poli , V. Raia , G. Tuccio , P. Vandamme
Italian Cystic Fibrosis Study Group, Palermo, Italy, Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium

A127: Novel strategy for the isolation of Burkholderia cepacia complex from the environment
E. Vanlaere, T. Coenye, E. Samyn, J. Govan, F. De Baets, K. De Boeck, C. Knoop, P. Vandamme
University of Ghent, Ghent, Belgium

A131: Factors affecting the survival rate in Burkholderia cenocepacia infected Czech CF patients.
D. Zemkova , P. Drevınek , J. Bartosova , L. Fila , V. Vavrova
Clinic of Pulmonology, Prague, Czech Republic

A183:CmaxSS-MIC ratio, as a predictor of CF patients infected with Burkholderia cenocepacia.
D. Zemkova , P. Drevinek , J. Bartosova , L. Fila , V. Vavrova
Paediatric Dept, 2nd Medical School, Prague, Czech Republic, Adult Pulmonology Dept, University Hospital Motol, Prague, Czech Republic

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Clinic Aspects

A154: In children with cystic fibrosis can bacteria isolated from spirometry filters guide clinical management? J.L. Hartley , J.H.M. Rees , M.C.J. Rudolf
Royal Shrewsbury Hospital, Shrewsbury, United Kingdom, Birmingham Children’s Hospital, Birmingham, United Kingdom, Leeds General Infirmary, Leeds, United Kingdom

A116: Chronological timing of respiratory pathogen acquisition in infants with CF in Northern Ireland.
C.A. Kavanagh, A.J.M. Reid
Royal Belfast Hospital For Sick Children, Belfast, United Kingdom

A184: The analysis of the isolated microorganisms from the respiratory tract of CF children treated in Children’s Memorial Health Institute 1999–2003.
K.S. Semczuk, H.D. Dmenska, D.D. Dzierzanowska, P.G. Gutkowski, M.M.Modrzewska, E.G. Gabinska, H.Z. Zareba
Warsaw, Poland

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Infection, Incidence and Diagnosis

A235: Unusual respiratory bacterial pathogens in a cystic fibrosis adult patients unit.
E. Inglezos, P. Boura, M. Kanellopoulou, M. Skarmoutsou, E. Papafrangas, F.Apostolopoulou.
Sismanoglio General Hospital, Maroussi-Athens, Greece

A139: Fingerprinting of cystic fibrosis sputum metagenome reveals unique amplicon length heterogeneity (ALH) profiles for each patient.
S. Miller , D. Mills , T. Pressler , C. Koch , K. Mathee
Florida International university, Department of Biological Sciences, Miami, United States, The National University Hospital (Rigshospitalet), Department of Pediatrics, Copenhagen, Denmark

A164: Identification of interacting groups of bacterial species colonising the CF lung, through statistical analysis of T-RFLP profiles.
G.B. Rogers , M.P. Carroll , P.M. Hockey , K.D. Bruce
Southampton General Hospital, Southampton, United Kingdom, King’s College London, London, United Kingdom

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Inquilinus limosus

A168: Inquilinus limosus: an unusual bacterium recovered in two French patients sputum with cystic fibrosis.
R. Chiron , F. Counil , F. Bremont , C. Segonds , E. Jumas-Bilak , H. Jean-Pierre , H. Marchandin
CHU Arnaud de Villeneuve, Montpellier, France, CHU Rangueil, Toulouse, France, Faculte de Pharmacie, Montpellier, France

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Pseudomonas Aeruginosa

A151: Pseudomonas aeruginosa in water of public outdoor and indoor swimming pools and bathroom water taps of patients with cystic fibrosis during the hot summer 2003.
J. Barben , G. Hafen , J. Schmid
Paediatric Pulmonology, Children’s Hospital, St. Gallen, Switzerland, Official Food Control Laboratory, St. Gallen, Switzerland

A175: Detection of volatile compounds released by Pseudomonas aeruginosa in vitro by selected ion flow tube mass spectrometry (SIFT-MS).
W.D. Carroll , D. Smith , T.S. Wang , P. Spanel , P.A. Alcock , W. Lenney
Academic Department of Paediatrics, University Hospital of North Staffordshire, Stoke-on-Trent, United Kingdom, Centre for Science & Technology in Medicine, Keele University, Keele, United Kingdom, J. Heyrovsky Institute of Physcial Chemistry, Academy of Sciences, Prague, Czech Republic, Department of Medical
Microbiology, University Hospital of North Staffordshire, Stoke-on-Trent, United Kingdom

A186: Real-time PCR detects non cultivable Pseudomonas aeruginosa in the early phase of CF lung colonisation.
G. Couetdic, F. El’Garch, J. Schneider, C. Millardet, M.L. Dalphin, J.C. Dalphin, P. Plesiat
Hopital Jean Minjoz, Besancon, France

A185: The Liverpool epidemic strain of Pseudomonas aeruginosa; 8 years after initial identification.
E.A. Gaillard , H.L. Wallace , L.J. Heaf , S. Panagea , P. Shears , E.F. Burrows , P. Garnett , J.J. Cottrell , M.J. Walshaw , M.J. Ledson , J.M. Couriel , R.L. Smyth , C.A. Hart , K.W. Southern , D.P. Heaf
Royal Liverpool Children’s Hospital, Liverpool, United Kingdom, Institute of Child Health, University of Liverpool, Liverpool, United Kingdom, Medical Microbiology, University of Liverpool, Liverpool, United Kingdom, Cardiothoracic Centre Adult CF Unit, Liverpool, United Kingdom

A134: Longitudinal surveillance for Pseudomonas aeruginosa cross-infection at an adult cystic fibrosis centre.
A.M. Jones , C.M. Smith , M.E. Dodd , C.J. Doherty , J.R.W. Govan , A.K. Webb
Manchester Adult Cystic Fibrosis Centre, Manchester, United Kingdom, Medical Microbiology, Edinburgh, United Kingdom

A439: Genotyping Pseudomonas aeruginosa isolates by pulsed field gel electrophoresis (PFGE) and randomly amplified polymorphic DNA (RAPD) techniques
D. Jyothish , M. Desai , N. West , P. Weller , J. Clarke , M. Mehmi , C. Powell , K. Hinton , A. Hilton , P. Lambert
Birmingham Children’s Hospital NHS Trust, Birmingham, United Kingdom, Aston University, Birmingham, United Kingdom

A155: Characterization of poxB, a chromosomal-encoded Pseudomonas aeruginosa oxacillinase.
K.F. Kong , S. Jayawardena , A. del Puerto , L. Wiehlmann , U. Laabs , B. Tuemmler , K. Mathee
Florida International University, Miami, United States, Klinische Forschergruppe, Medizinische Hochschule Hannover, Hannover, Germany

A167: Maintenance of high adherence capacity of Pseudomonas aeruginosa cystic fibrosis isolates during chronic infection is associated with more severe lung disease.
U. Laabs , P. Gudowius , L. Wiehlmann , A.S. Limpert , A. Filloux , Tummler , S. de Bentzmann
CNRS IBSM UPR9027, Marseille, France, Medizinische Hochschule Hannover, Hannover, Germany, Universitats kinderklinik, Dusseldorf, Germany

A136: Identification and prevalence of DNA markers specific to a multiresistant and transmissible clone of Pseudomonas aeruginosa
D.A. Miller , A.M. Jones , A.K. Webb , J.R.W. Govan , D.P. Speert , C. Smith E. Mahenthiralingam
Cardiff School of Biosciences, Cardiff University, Cardiff, United Kingdom, Bradbury CF Unit, Wythenshawe Hospital, Manchester, United Kingdom, Medical Microbiology, University of Edinburgh, Edinburgh, United Kingdom, Dept. of Paediatrics, University of British Columbia, Vancouver, Canada

A156: Effect of pH on inorganic polyphosphate accumulation in Pseudomonas aeruginosa and Burkholderia cepacia isolates from patients with cystic fibrosis.
T.F. Moriarty , C. Laverty , A. Mullan , J. McGrath , J.S. Elborn , M.M. Tunney
School of Biology and Biochemistry, Queen’s University, Belfast, United Kingdom, Respiratory Medicine Research Group, Queen’s University, Belfast, United Kingdom, Belfast City Hospital, Belfast, United Kingdom

A153: Domestic washing machines: a reservoir for Pseudomonas aeruginosa?
T.L. Pitt, H. Englender, H.M. Aucken.
Laboratory of HealthCare Associated Infection, SRMD,HPA Colindale, London,United Kingdom

A133: Use of multilocus restriction typing method to detect hypertransmissible P. aeruginosa strains from patients attending Cardiff CF clinics.
H.C. Ryley , J.L. Weeks , D.J. Shale , I.J.M. Doull
Dept of Medical Microbiology, UWCM, Cardif, United Kingdom, Section of Respiratory Disease, UWCM, Penarth, United Kingdom, Dept of Child Health, Cardiff, United Kingdom

A135: Characterisation of a diagnostic probe for the Pseudomonas aeruginosa Liverpool CF epidemic strain. C.H. Smart, C.A. Hart, C. Winstanley
University of Liverpool, Liverpool, United Kingdom

A137: Clinical consequences of transmissible Pseudomonas aeruginosa infection in patients with cystic fibrosis—a 4 year prospective study
C.M. Smith, M.E. Dodd, A.K. Webb, A.M. Jones
Wythenshawe Hospital, Manchester, United Kingdom

A161: Effect of growth under anaerobic conditions on the antimicrobial susceptibility of Pseudomonas aeruginosa isolates from cystic fibrosis patients.
M.M. Tunney , A. White , J.S. Elborn
Respiratory Medicine Research Group, Queen’s University, Belfast, United Kingdom, Belfast City Hospital, Belfast, United Kingdom

A440: Newly acquired Pseudomonas aeruginosa (P.a.) in Belgian CF patients:does the patients’ P.a. genotype correlate with environmental genotypes?
S. Van daele , F. De Baets , P. Schelstraete , L. Van Simaey , G. Claeys , M. Vaneechoutte
Pediatric Pulmonology,University Hospital, Gent, Belgium, Microbiology,University Hospital, Gent, Belgium

A443:
Pseudomonas aeruginosa DNA-fingerprinting register in Belgian CF patients: a one year survey
S. Van daele , M. Vaneechoutte , G. Claeys , P. Schelstraete , F. Haerynck , F. De Baets
Pedaitric Pulmonology,University Hospital, Gent, Belgium, Microbiology,University Hospital, Gent, Belgium

A174: Comparative proteomic analysis of planktonic and biofilm Pseudomonas aeruginosa cells: identification of differentially expressed proteins.
S. Vilain, P. Cosette, D. Seyer, G.A. Junter, T. Jouenne.
UMR 6522 CNRS, IFRMP 23, Mont-Saint-Aignan, France

A176: DNA chip for geno- and pathotyping of Pseudomonas aeruginosa
L. Wiehlmann , G. Morales , B. Siebert , G. Wagner , P. Slickers , B. Tummler
Klinische Forschergruppe, OE 6710, Medizinische Hochschule Hannover, Hannover, Germany, Centro Nacional de Biotecnologia, Madrid, Spain, Clondiag Chip Technologies, Jena, Germany

A46: Pseudomonas aeruginosa adherence to murine nasal epithelium
T. Wodehouse , R. Farley , B. Wainwright , D.M. Geddes , J.C. Davies , E.W.F.W. Alton
Department of Gene Therapy at Imperial College London, UK CFGT Consortium, London, United Kingdom, Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia

A50: Binding of Pseudomonas aeruginosa to cystic fibrosis human nasal
epithelium
T. Wodehouse, L.A. Collins, D.M. Geddes, J.C. Davies, E.W.F.W. Alton
Department of Gene Therapy, Imperial College; UK CFGT Consortium, London,United Kingdom

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Staphylococcus Aureus

A163: Fas-induced apoptosis of Staphylococcus aureus-infected airway epithelial cells 
M.C.A. Da Silva , M.C. De Assis , J.-M. Zahm , E. Puchelle , M.C. Plotkowski
Institut of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil, Department of Microbiology and Immunology, State University of Rio de Janeiro, Rio de Janeiro, Brazil, INSERM U314, Reims, France.

A165: Staphylococcus aureus and airway epithelial cell interaction.
D. Gras , M. Da Silva , M. Milliot , J.M. Zahm , O. Bajolet , M.C. Plotkowski , E. Puchelle INSERM UMRS 514, Reims, France, Laboratoire de Bacteriologie-Virologie-Hygiene, CHU, Reims, France, Department of Microbiology, University of Rio Janeiro, Rio Janeiro, Brazil

A148: Risk factors for MRSA colonization in patients with cystic fibrosis. 
K. Nadesalingam , S.P. Conway , M. Denton
Department of Microbiology, Leeds Teaching Hospitals, Leeds, United Kingdom, Regional Cystic Fibrosis Unit, Leeds Teaching Hospitals, Leeds, United Kingdom

A149: Methicillin-resistant Staphylococcus aureus (MRSA) in French CF patients : an epidemiologic approach.
C. Segonds , P. Clavel-Batut , K. Eydieux-Laporte , I. Le Thomas-Bories , E. Bingen , P. Mariani , N. Brahimi , S. Ravilly , G. Chabanon
Hopital Rangueil, Toulouse, France, Hopital Robert-Debre, Paris, France, Vaincre la Mucoviscidose, Paris, France

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Stenotrophomonas Maltophilia

A172: Increasing prevalence of Stenotrophomonas maltophilia in patients with cystic fibrosis.
J. Larsson Friden, M. Gilljam, B. Jonsson
Sahlgrenska University Hospital, Goteborg, Sweden ¨

Virology

A118: Does respiratory viral infections influence lung function and bacterial colonisation in children with CF? H.V. Olesen , L.P. Nielsen , P.O. Schiotz
CF-centre West, Aarhus University Hospital, Skejby Sygehus, Aarhus, Denmark, Department of Clinical Microbiology, Odense University Hospital, Odense, Denmark

A247: Respiratory viruses in cystic fibrosis
D.S. Wat , C.M. Gelder , S. Hibbitts , I. Bowler , R. Evans , M. Pierrepoint , I.J. Doull
University of Wales College of Medicine, Cardiff, United Kingdom, Llandough Hospital, Penarth, United Kingdom, Royal Gwent Hospital, Newport, United Kingdom, Singleton Hospital, Swansea, United Kingdom, University Hospital ofWales, Cardiff, United Kingdom

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